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    Plant Phosphatidic acid ELISA kit

    發(fā)布時間: 2010-04-19  點擊次數(shù): 1986次

     

    INTENDED USE
    The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
     
    PRINCIPLE OF THE METHOD
    The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
     
    REAGENTS PROVIDED AND RECONSTTTUTION

    REAGENTS(Store at 2-8℃)
    1×96 WELLS
    0.5×96 WELLS
    RECONSTTTUTION
    96/48-wells microtiter plates
    1
    0.5
    Ready-to-use
    Plastiv cover
    2
    1
    Ready-to-use
    Standard: 40ng/ml
    1Vials (0.6ml)
    0.5Vials (0.3ml)
    See reagents preparation on page 3
    Blank control
    1Vials (1.0ml)
    1Vials  (0.5ml)
    Ready-to-use
    Standard Diluent
    1Vials (5ml)
    1Vials   (2.5ml)
    Ready-to-use
    Biotinylated anti-PA
    1Vials (6ml)
    1Vials   (3.0ml)
    Ready-to-use
    Streptavidin-HRP
    1Vials (10ml)
    1Vials   (5.0ml)
    Ready-to-use
    Washing Buffer
    1Vials (60ml)
    1Vials   (30ml)
    20× concentrate
    Substrate  A
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Substrate  B
    1Vials 6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Stopping Solution
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use

     
    MATERIAL REQUIRED BUT NOT PROVIDED
    l       Distilled water
    l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
    l       Vortex mixer and magnetic stirrer.
     
    INTENDED USE
    The PA ELISA is to be used for the quantitative determination of PA. The assay will recognize both natural and recombinant PA. The kit has been configured for research use only and is not to be used in diagnostic procedures.
     
    PRINCIPLE OF THE METHOD
    The PA kit is a solid phase phase sandwich enzyme linked immuno sorbent assay(ELISA). Samples , including standards of known PA concentrations and unknowns are pipetted into these wells. During the first incubation, the PAantigen and a biotinylated monoclonal antibody specific for PAare simultaneously incubated. After washing, the enzyme(streptavidin-peroxydase)is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of PApresent in the samples.
     
    REAGENTS PROVIDED AND RECONSTTTUTION

    REAGENTS(Store at 2-8℃)
    1×96 WELLS
    0.5×96 WELLS
    RECONSTTTUTION
    96/48-wells microtiter plates
    1
    0.5
    Ready-to-use
    Plastiv cover
    2
    1
    Ready-to-use
    Standard: 40ng/ml
    1Vials (0.6ml)
    0.5Vials (0.3ml)
    See reagents preparation on page 3
    Blank control
    1Vials (1.0ml)
    1Vials  (0.5ml)
    Ready-to-use
    Standard Diluent
    1Vials (5ml)
    1Vials   (2.5ml)
    Ready-to-use
    Biotinylated anti-PA
    1Vials (6ml)
    1Vials   (3.0ml)
    Ready-to-use
    Streptavidin-HRP
    1Vials (10ml)
    1Vials   (5.0ml)
    Ready-to-use
    Washing Buffer
    1Vials (60ml)
    1Vials   (30ml)
    20× concentrate
    Substrate  A
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Substrate  B
    1Vials 6.0ml)
    1Vials   (3.0ml)
    Ready-to-use
    Stopping Solution
    1Vials (6.0ml)
    1Vials   (3.0ml)
    Ready-to-use

     
    MATERIAL REQUIRED BUT NOT PROVIDED
    l       Distilled water
    l       Pipettes:10ul、50ul、100ul、200ul、1000ul。
    l       Vortex mixer and magnetic stirrer.
     
     
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